Analytical Biochemistry Services Offered

Analysis

  1. 1-hydroxy-pyrene in urine
  2. 5-Nitro-gamma tocopherol in plasma
  3. Acids (citrate cycle analytes): lactic acid, pyruvic acid, alpha-ketoglutaric acid, from liver tissue
  4. Aldehydes (formaldehyde, acetaldehyde, acrolein) by LCMS
  5. Alkaloids from urine, saliva, hair, plants by LCMS (see nicotine, cotinine, Areca alkaloids)
  6. Alkylresercinols from serum
  7. Ammonia in urine
  8. APO-A1 in plasma by AA
  9. Apo-B in plasma by AA
  10. Apo-E in plasma by AA
  11. Areca (Betel) alkaloids from urine, saliva, plants by LCMS (arecoline, arecaidine, guvacine, guvacoline)
  12. Bisphenol-A in urine by LC/MSMS
  13. Caffeine and its metabolites in urine
  14. Calcium in plasma by AA
  15. Carotenoids in the eye by macular pigment optical density reading using heterochromatic flicker spectrophotometry
  16. Carotenoids including their isomers in plasma by HPLC/PDA (luteins, zeaxanthins, alpha- and beta-cryptoxanthins, lycopenes, alpha- and beta-carotenes)
  17. Chavibetol from urine, saliva, plants by LCMS
  18. Cholesterol (total) in plasma by AA
  19. Cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, ursodeoxycholic acid, and hyodeoxycholid acid from serum
  20. Choline, betaine, carnitine, trimethylamine-N-oxide from plasma or serum (TMAO) by LCMS
  21. Choline, betaine, CDP-choline, phosphocholine, glycerophosphocholine from liver tissue
  22. Citric acid, and the most abundant fatty acids from liver tissue
  23. Coenzyme Q10 (ubiquinol-10 and ubiquinone-10) by HPLC/PDA
  24. Cotinine and hydroxycotinine from saliva;
  25. Hydroxylated cholesterols in plasma by LCMS
  26. Coenzyme Q10 in plasma by HPLC/ECD (oxidized, reduced and total Q10)
  27. Creatinine in urine by AA
  28. CRP in plasma by AA
  29. DNA by HPLC
  30. DNA methylation, global (5-methyl-dC/dG)
  31. Fatty acids in plasma or RBC's by high-accuracy LCMS (myristic acid [MA, C14:0], stearic acid [SA, C18:0], palmitic acid [PA, C16:0], oleic acid [OA, C18:1], palmitoleic acid [PLA, C16:1], docosapentaenoic acid [DPA, C22:5 n-3], docosahexaenoic acid [DHA, C22:6 n-3], eicosapentaenoic acid [EPA, C20:5 n-3], α-linolenic acid [ALA, C18:3 n-3], eicosadienoic acid [EDA, C20:2 n-6], linoleic acid [LA, C18:2 n-6], arachidonic acid [AA, C20:4 n-6], α-eleostearic acid [αESA, C18:3]; stearic acid-d3 [SA-d3], linoleic acid-d4 [LA-d4], arachidonic acid-d8 [AA-d8], palmitoleic acid-d14 [PLA-d14], Eicosapentaenoic acid-d5 [EPA-d5], docosahexaenoic acid-d5 [DHA-d5], or any other fatty acid)
  32. Folates in plasma by LC/MSMS
  33. Glyphosate (Roundup), glufosinate and AMPA from urine by LCMS
  34. Haptoglobin in plasma by AA
  35. HDL-cholesterol in plasma by AA
  36. Homocysteine, cysteine in plasma by LCMS
  37. Hydroxylated cholesterols in plasma by LCMS
  38. Isoflavonoids, flavonoids, lignans, anthocyanins or other groups of phenolic agents from plasma, urine, breast milk, foods by LC/MSMS
  39. Isothiocyantes (ITC) from plasma or serum by LCMS (allyl-ITC, butenyl-ITC, phenethyl-ITC, iberin, sulforaphane)
  40. L-alanine, L-glutamic acid, L-glutamine, glucose-6-phosphate from liver tissue
  41. Lipid panel in plasma by AA (Chol, HDLC, triglycerides)
  42. Lipid-Phase Micronutrients in plasma or foods by HPLC/PDA/FD (12 carotenoids, 2 retinoids, 3 tocopherols)
  43. Macular pigment optical density by heterochromatic flicker spectrophotometry (using a model Macular Metrix II from Macular Metrics)
  44. Metabolomics by LCMS
  45. Methylmalonic acid in plasma by LC/MSMS
  46. Neurotransmitters in CSF or plasma/serum by HPLC/FD/ECD: norepinephrine, 5-hydroxyindole-3-acetic acid, homovanillic acid, kynurenic acid, L-kynurenine, tryptophane
  47. Nicotine and metabolites from urine, saliva, hair, plants by LCMS (nicotine, nornicotine, anabasine, myosmine, cotinine, hydroxycotinine, NNN [N-nitrosonornicotine], NNK [4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone], NAB [N-nitrosoanabasine], and NAT [N-nitrosoanatabine])
  48. Nitrogen, urea in urine by AA
  49. Oxytocin from serum by LCMS
  50. Parabens from urine by LCMS
  51. Phenoxyacetic acids (2,4-D; 2,4-T) from urine by LCMS
  52. Phospholipids (the most abundant arrays of phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, lysophosphatidylcholines, and phosphatidylinositols) from liver tissue
  53. Phthalates in urine by LC/MSMS (phthalate, monoethyl phthalate, mono-n-butyl phthalate, mono-isobutyl phthalate, monobenzyl phthalate, mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-hydroxyhexyl) phthalate, mono(2-ethyl-5-oxohexyl) phthalate, mono(2-ethyl-5-carboxypentyl) phthalate)
  54. Red blood cell preparation
  55. Roundup (glyphosate) and aminomethyl phosphonic acid (AMPA) from urine by LCMS
  56. Sucrose, fructose, glucose in urine by LC/MSMS
  57. Total phenol levels in food, urine, plasma
  58. Triclosan from urine by LCMS
  59. Triglycerides in plasma by AA**
  60. Trimethylamino-N-oxide (TMAO), choline, betaine, and carnithine from serum
  61. Vanillin from e-cigarette juices by LCMS
  62. Vitamin A and pro-vitamin A in plasma by HPLC/PDA
  63. Vitamin B group in plasma by LC/MS (pyridoxic acid, pyridoxal phosphate; folate, 5-methyltetrahydrofolate; methylmalonic acid; homocysteine, cysteine; choline, betaine)
  64. Vitamin C in plasma, food by HPLC/ECD or PDA
  65. Vitamin D in plasma by LC/MSMS (vitamin D2, vitamin D3, 25-hydroxy-vitamin D2, and 25-hydroxy-vitamin D3)
  66. Vitamin E in plasma by NP-HPLC/PDA/FD (all tocopherols)
  67. Vitamin K by LC/MSMS
  68. Vitamin and antioxidant panel (lipid soluble) from plasma by single HPLC/PDA/FD (25-hydroxy-vitamin D2 and 25-hydroxy-vitamin D3; alpha-, beta-, gamma-, and delta-tocopherol; all-trans and geometric isomers of lutein, zeaxanthin, alpha- and beta-cryptoxanthin, lycopene, and alpha- and beta-carotene; retinol and retinyl palmitate; ubiquinol-10 and ubiquinone-10)
  69. Steroids in urine and plasma/serum by LC/MS (estriol [E3], 16-epiestriol [16epi-E3], 17-epiestriol [17epi-E3], 16-ketoestradiol [16keto-E2], 16-α-hydroxyestrone [16αOH-E1], 2-methoxyestrone [2MeO-E1], 3-methoxyestrone [3Me-OE1], 4-methoxyestrone [4MeO-E1], 2-methoxyestradiol [2MeO-E2], 4-methoxyestradiol [4MeO-E2], 2-hydroxyestrone [2OH-E1], 4-hydroxyestrone [4OH-E1], 2-hydroxyestradiol [2OH-E2], progesterone [P], testosterone [T]), corticoids (cortisol, cortisone, etc.) and other steroids
  70. Any other assay based on clinical autoanalyzer technique (Cobas MiraPlus CC)

Enzyme immunoassays

  1. 25 Hydroxy Vitamin D
  2. Adiponectin
  3. Interleukin-2
  4. Interleukin-6
  5. Interleuken-6 soluble Receptor
  6. Leptin
  7. Parathyroid Hormone
  8. TNF alpha
  9. TNF alpha Receptor I
  10. TNF alpha Receptor II
  11. Urinary Isoprostane
  12. Any other ELISA based assay
  13. Any Luminex based ELISA assay

Equipment use

  1. HPLC/PDA systems (no supplies)
  2. LC/MS system (no supplies)

Consultation

  1. technical (staff)
  2. professional (director)
  3. lab experiments (total analysis including supplies)

All plasma assays can be done from serum.

All other ELISA assays can be performed upon request. Please contact Dr. Franke, This email address is being protected from spambots. You need JavaScript enabled to view it..

  1. AA = clinical chemistry auto analyzer (Cobas MiraPlus CC)
  2. ECD = electrochemical detection
  3. FD = fluorescence detection
  4. HPLC = high pressure liquid chromatography
  5. LC/MS = liquid chromatography mass spectrometry
  6. LC/MSMS = liquid chromatography tandem mass spectrometry
  7. NP = normal-phase
  8. PDA = photo diode array detection
  9. RBC = red blood cells

Resources and Instrumentation in this laboratory

  1. 3 fully automated liquid chromatography mass spectrometry (LC/MS) systems: 2 orbitrap (model Q-Exactive, Thermo, Woburn MA) and 1 triple quadrupole instrument (model TSQ Ultra, Thermo), all equipped with electrospray or atmospheric pressure chemical ionization or atmospheric pressure photo ionization, all allowing positive and negative ion monitoring. LC models are all Accela (Thermo)
  2. 3 fully automated high pressure liquid chromatography (HPLC) systems (model Surveyor, Thermo) and 2 ultra high pressure liquid chromatography systems (UHPLC; model Accela, Thermo, attached to the mass spectrometers above), all with quaternary pumps that can all be connected to above MS systems or/and to one of 5 available photo-diode array (PDA) detectors or to fluorescence (FL; model FD100, GTI/SpectroVision, Concord, MA) or electrochemical detectors (Coulochem III with 5021 dual cell or Coularray with 8 cells, ESA, Chelmsford, MA) devices
  3. 1 Macular Pigment Optical Density (MPOD) reader based on heterochromatic flicker spectrophotometry (Macular Metrix II, Macular Metrics, MA)
  4. 2 nitrogen generators (Peak Scientific, Billerica, MA)
  5. 1 post column derivatization system (Timberline Co., Boulder, CO)
  6. For routine ELISA assays: 2 model 'VersaMax' microplate reader with 'Analyst AD' capabilities for UV/fluorescence readings (Molecular Devices, Sunnyvale, CA) and one Luminex200 instrument (Luminex Corpor., Austin, TX) for multiplexing immunoassays
  7. 1 spectrophotometer (model BioSpec160I, Shimadzu Co., Columbia, MD)
  8. 2 clinical autoanalyzers (Roche Cobas Mira Plus CC, Roche Diagnostics Inc., Indianapolis, IN)
  9. 1 liquid handling robot (model Versa 100, Aurorabiomed, Vancouver, BC)
  10. 2 rotating evaporators (Büchi, Switzerland)
  11. 2 centrifuges (IEC Co., Vermont Hill, IL),
  12. 2 8ºC refrigerators
  13. 1 -4ºC freezer
  14. 1 -20ºC freezer
  15. 2 -80ºC freezers (Fisher Scientific Co., VWR Scientific Co.)
  16. 3 biological fume hood with laminar flow (Labconco, Kansas City, MO)
  17. one 4 TB backup system for data storage and archiving

Other equipment this operation has access to includes a solid-phase extraction system, nitrogen gas evaporation system, rotary evaporation system, and a complete Millipore water filter system with ion exchange columns (all from Waters, Milford, MA). Additional shared equipment includes a Beckman L2-65B and a L8-70M ultracentrifuges (SW55 Ti, SW27, 70 Ti, SW41 rotors), Sorvall OTD-50 ultracentrifuge (TV865 rotor), a lyophilizer (Labconco), a Beckman LS 7500 and LS100 liquid scintillation counters, a Beckman Gamma 5500B counter, a model AS 160 speed-vac (Savant, Farmingdale, NY) for evaporation of small multiple samples, five ultra-low temperature freezers (Forma Scientif. Co., Queue Systems, Revco Scientific Co., Asheville, NC), a Coulter automatic cell counter, a fluorometer (Perkin-Elmer Co., Cupertino, CA), and several sterilizers including a high pressure vapor sterilizer.

Updated: 03/23/2018

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