Chemical Biology Core Research Facility

Compound Screening Facility

Overview

The Screening Core Facility is focused on evaluating natural products and small-molecules against validated cancer targets and pathways that are the focus of UH Cancer Center faculty and researchers. The centralized core facility will facilitate the screening process to identify new lead compounds for further biochemical and biological studies. Working with medicinal chemists, these efforts are directed toward the ultimate goal of discovery and development of suitable chemical probes with potential therapeutic application.

Assays and Screening activities

The Screening Core houses the UH Cancer Center's Natural Products Library, a collection of samples from the endemic species in the Hawaiian Islands. Natural product chemists are involved in the maintenance of cyanobacteria cultures, extracts preparation and fractionation, and the purification and identification of bioactive and other components. Synthetic and medicinal chemists, and biologists are involved in the evaluation of samples and lead optimization efforts. Investigators are developing in vitro and cell-based smart assays that are used to identify bioactive natural product compounds capable of selectively and potently altering key cancer targets. Several medium throughput screening assays using modern robotics equipment are being developed as part of the Compounds Screening Core Facility. Target-focused assays include Stat3, RSK, ion channels (offered through the sister core at The Queen's Medical Center; http://qcbr.jabsom.hawaii.edu/HTS/index.html), p53, HMGB1, Fructose transport, Bile acid transport, Aurora A, and receptor tyrosine kinases. Other assays focus on signaling pathways, such as integrin signaling.

Nuclear Magnetic Resonance (NMR) Instrumentation

The Chemical Biology Core Research Facility houses a 600 MHz NMR spectrometer that is available for small-molecule and protein work. All common 2D routines are available as well as the assistance of a skilled operator to set up experiments, collect and display data and to contribute to the analysis and interpretation of the results, if necessary. This new (in 2017) high field spectrometer represents an important upgrade of the university's research infrastructure that leverages many years of experience within the Chemistry Department.

 

 

PerkinElmer Envision Multimode Plate Reader
PerkinElmer Envision
Multimode Plate Reader
BioMek NXP-96 Channel
BioMek NXP-96 Channel
BioMek NXP-8 Channel
BioMek NXP-8 Channel